NOVON 7(2): 93-94. 1997.

Arabidopsis gamosepala and A. tuemurnica Belong to Neotorularia (Brassicaceae)

Ihsan A. Al-Shehbaz

Missouri Botanical Garden, P.O. Box 299, St. Louis, Missouri 63166-0299, U.S.A.

 Steve L. O’Kane, Jr.

Department of Biology, University of Northern Iowa, Cedar Falls, Iowa 50614-0421, U.S.A.

ABSTRACT. The new combination Neotorularia gamosepala is proposed. Arabidopsis tuemurnica is reduced to synonymy of N. humilis. The limits of Arabidopsis and Neotorularia are discussed.

Although Arabidopsis thaliana has become the plant of choice in molecular, developmental, physiological, genetic, and related studies, little is known about its immediate relatives, and the limits of Arabidopsis remain controversial (Al-Shehbaz, 1988; Price et al., 1994). For the past few years, the present authors have been working on the elucidation of the generic boundaries of Arabidopsis, and some of the species previously assigned to this genus have already been transferred to other genera (Al-Shehbaz, 1994; Al-Shehbaz & O’Kane, 1995). The present paper deals with two such controversial species.

On the basis of molecular studies (O’Kane et al., 1997), Arabidopsis gamosepala Hedge (endemic to Afghanistan) and A. tuemurnica Kuan & An (endemic to China) form with the widespread Neotorularia torulosa (Desfontaines) Hedge & Léonard (China west into western Russia, the Middle East, and North Africa) a well-defined clade distinctly unrelated to that including Arabidopsis. The evidence from morphology (see below) fully supports the molecular data to include these two species in Neotorularia.

Schulz (1924) separated Arabidopsis from Torularia (now Neotorularia) primarily on the basis of having mucilaginous instead of non-mucilaginous seeds. However, this feature is unreliable, and many genera of the Brassicaceae include species with or without seed mucilage (Vaughan & Whitehouse, 1971). The most reliable characters for the separation of these genera are the trichome and fruit types. Neotorularia has distinctly torulose fruits with pubescent valves, and the trichomes are primarily few to several branched. In contrast, Arabidopsis has non-torulose glabrous fruits, and the trichomes are simple mixed primarily with 2-forked ones. Both Arabidopsis gamosepala and A. tuemurnica have the typical features of Neotorularia mentioned above.

A synopsis of Neotorularia is being carried out by the authors, and it is estimated that the genus includes 25--30 species. Because the completion of the revision will take some time, the following nomenclatural adjustments are proposed to make them available for some of the checklists and floras in progress, especially the forthcoming treatment of the Brassicaceae for the Flora of China.

Neotorularia gamosepala (Hedge) Al-Shehbaz & O’Kane, comb. nov. Basionym: Arabidopsis gamosepala Hedge, Fl. Iran. 57: 334. 1968. TYPE: Afghanistan. Munjan: above Anjuman valley, near Anjuman, 3100 m, 14 Aug. 1965, Podlech 12379 (holotype, M; isotype, W).

Hedge (1968) indicated that Arabidopsis gamosepala has no clear ally in Arabidopsis and that it is anomalous in the genus for lacking the median nectar glands, a feature characteristic of Neotorularia. The species appears to be most closely related to N. torulosa, from which it is readily distinguished in being a perennial with a gamosepalous calyx. Neotorularia torulosa is an annual with free sepals.

Neotorularia humilis (C. A. Meyer) Hedge & Léonard, Bull. Jard. Bot. Belg. 56: 394. 1986. Sisymbrium humile C. A. Meyer, in Ledebour, Icon. Pl. Ross. 2: 16. 1830. TYPE: Altai, C. A. Meyer s.n. (holotype, LE).

Arabidopsis tuemurnica Kuan & An, Bull. Bot. Lab. North-East. Forest. Inst. 8: 44. 1980. Syn. nov. TYPE: China. Xinjiang: Wen-su Xian, Tuo-mu-er-feng, 2400 m, 24 June 1977, Tuo-mu-er-feng Expedition 770084 (holotype, PE, listed as HP; isotype, BJM, listed as HM).

In their original description of Arabidopsis tuemurnica, Kuan and An (1980) compared the species with A. thaliana and indicated that it is an annual differing primarily in the basal leaves. However, an examination of the holotype of the former reveals that it is a short-lived perennial clearly unrelated to A. thaliana, especially in fruit and trichome characters. In fact, the plant is indistinguishable from the highly variable Neotorularia humilis, a species widespread in northern North America (Alaska and Canadian Arctic south to Colorado and east to Vermont) and northern Asia (Russia and China south to the Himalayas and west to Afghanistan, Pakistan, and Central Asia). North American authors (e.g., Abbe, 1948; Harris, 1985; Rollins, 1993) treated N. humilis in Braya. However, the molecular evidence (O’Kane et al., 1997; O’Kane, unpublished), as well as the critical morphological comparison of the species with its Asian representatives and other Neotorularia species, clearly support the placement of this species in Neotorularia. Unfortunately, all studies of the North American Braya completely ignored the Asian members and gave no reference to Neotorularia or Torularia. In our opinion, the North American Braya needs a comprehensive study, and some of its species may well prove to be Neotorularia, a genus up to this study not yet recognized in North America.

Acknowledgments.

We are grateful to Barbara Schaal (Washington University, St. Louis) for providing the space and facilities in her lab to conduct molecular studies on various genera of the Brassicaceae. The senior author gratefully acknowledges the generous financial support of the National Science Foundation (grant DEB-9208433) and the National Geographic Society (grant 5068-93) to conduct fieldwork in Europe and Asia.

Literature Cited

Abbe, E. C. 1948. Braya in Boreal eastern America. Rhodora 50: 1--15.

Al-Shehbaz, I. A. 1988. The genera of Sisymbrieae (Cruciferae; Brassicaceae) in the southeastern United States. J. Arnold Arbor. 69: 213--237.

---. 1994. Erysimum hedgeanum (Brassicaceae), a new name replacing Arabidopsis erysimoides. Novon 4: 1--2.

--- & S. L. O’Kane, Jr. 1995. Placement of Arabidopsis parvula in Thellungiella (Brassicaceae). Novon 5: 309--310.

Harris, J. G. 1985. A Revision of the Genus Braya (Cruciferae) in North America. Unpublished Ph.D. thesis, University of Alberta, Canada.

Hedge, I. C. 1968. Arabidopsis. In: K. H. Rechinger (editor), Fl. Iran. 57: 328--334.

Kuan, K. C. & Z. X. An. 1980. Taxa nova cruciferarum sinicarum. Bull. Bot. Lab. North-East. Forest. Inst. 8: 39--46.

O’Kane, S. L., Jr., B. A. Schaal & I. A. Al-Shehbaz. 1996 [1997]. The origins of Arabidopsis suecica (Brassicaceae) as indicated by nuclear rDNA sequences. Syst. Bot. vol. 21 (in press).

Price, R. A., J. D. Palmer & I. A. Al-Shehbaz. 1994. Systematic relationships of Arabidopsis: A molecular and morphological perspective. In: E. M. Meyerowitz & C. R. Sommerville (editors), Arabidopsis. Cold Springs Harbor Laboratory Press, New York.

Rollins, R. C. 1993. The Cruciferae of Continental North America. Stanford Univ. Press, Stanford, California.

Schulz, O. E. 1924. Cruciferae--Sisymbrieae. In: A. Engler, Pflanzenr. IV 105(Heft 86): 1--388.

Vaughan, J. G. & J. M. Whitehouse. 1971. Seed structure and the taxonomy of the Cruciferae. Bot. J. Linn. Soc. 64: 383--409.